Accurate prediction of gene feedback circuit behavior from component properties. Rosenfeld, N., Young, J.W., Alon, U., Swain, P.S. Refinement and standardization of synthetic biological parts and devices. Toward rational design of bacterial genomes. Automated design of synthetic ribosome binding sites to control protein expression. Successes and failures in modular genetic engineering. Contextualizing context for synthetic biology-identifying causes of failure of synthetic biological systems. Manufacturing molecules through metabolic engineering. Creation of a bacterial cell controlled by a chemically synthesized genome. DNA assembly for synthetic biology: from parts to pathways and beyond. The second wave of synthetic biology: from modules to systems. We expect the genetic element definitions validated here can be collectively expanded to create collections of public-domain standard biological parts that support reliable forward engineering of gene expression at genome scales.Įndy, D. We studied all combinations of curated elements, demonstrating that arbitrary genes are reliably expressed to within twofold relative target expression windows with ∼93% reliability. We measured activity distributions for each library and selected elements that collectively resulted in expression across a 1,000-fold observed dynamic range. We engineered libraries of constitutive and repressor-regulated promoters along with translation initiation elements following these definitions. We developed an expression cassette architecture for genetic elements controlling transcription and translation initiation in Escherichia coli: transcription elements encode a common mRNA start, and translation elements use an overlapping genetic motif found in many natural systems. An inability to reliably predict quantitative behaviors for novel combinations of genetic elements limits the rational engineering of biological systems.
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